Serologically Defined Colon Cancer Antigen 1 (SDCCAG1)

By SEREX (serologic analysis of recombinant cDNA expression libraries), Scanlan et al. (1998) cloned SDCCAG1, which they called NY-CO-1, from a colon cancer cDNA library. The deduced 362-amino acid protein contains a nuclear targeting signal.

The screening of cDNA expression libraries derived from human tumors with autologous antibody (SEREX) has proven to be a powerful method for defining the structure of tumor antigens recognized by the humoral immune system. In the present study, 48 distinct antigens (NY-CO-1-NY-CO-48) reactive with autologous IgG were identified by SEREX analysis in 4 patients with colon cancer. Sequencing analysis showed that 17 of the cDNA clones were previously uncharacterized molecules and 31 represented known gene products.