Shroom Family, Member 3 (SHROOM3)

SHROOM3 is necessary and sufficient to induce a redistribution of the microtubule regulator gamma-tubulin .This mutation was designated 'shroom' (shrm), because the neural folds 'mushroom' outward and do not converge at the dorsal midline. By 5-prime RACE and library screening, the cDNA for the trapped gene was cloned. Sequence analysis indicated there are at least 2 shrm transcripts that encode 2 different putative protein products. The longest predicted transcript, shrmL, encodes a protein of 1,986 amino acids, while the second transcript, shrmS, encodes a protein of 1,808 amino acids that lacks the N-terminal 177 amino acids of shrmL. Shrm is a PDZ domain-containing protein related to Xenopus Apx and human APXL (SHROOM2).