Shugoshin Like Protein 1 (SGOL1)

Endogenous HeLa cell SGOL1 was degraded by both CDC20- and CDH1-activated anaphase-promoting complex (APC), resulting in loss of SGOL1 from kinetochores during the metaphase-to-anaphase transition. SGOL1 depletion by RNA interference (RNAi) caused mitotic arrest with loss of sister kinetochore cohesion. Chromosomes and their centromeres did not align to form a metaphase plate; instead they scattered throughout the cell. SGOL1 depletion also destabilized kinetochore microtubules.

SGOL1 is required for mitotic progression and chromosome segregation and provides a link between sister centromere cohesion and microtubule interactions at kinetochores.