Solute Carrier Family 17, Member 9 (SLC17A9)

SLC17A9, which encodes a deduced 430-amino acid protein with 12 transmembrane domains. Northern blot analysis of mouse and human tissues detected wide SLC17A9 expression, with highest levels in adrenal gland, brain, and thyroid gland. Immunohistochemical analysis localized Slc17a9 to chromaffin granules in mouse adrenal medulla. SDS-PAGE of human SLC17A9 expressed in insect cells revealed proteins of 63 and 68 kD. Proteoliposomes containing purified human SLC17A9 actively took up ATP, ADP, and GTP using membrane potential as the driving force. ATP uptake was Cl- dependent, and the properties of ATP transport were similar to those found in synaptic vesicles and chromaffin granules. Suppression of endogenous Slc17a9 in a rat pheochromocytoma cell line decreased exocytosis of ATP.