Solute Carrier Family 25, Member 32 (SLC25A32)

Folate metabolism is distributed between the cytosolic and mitochondrial compartments. SLC25A32 is a transporter that shuttles folates from the cytoplasm into mitochondria. The deduced 315-amino acid protein has a calculated molecular mass of 35.4 kD. It has 6 transmembrane domains, 3 interspersed loops facing the mitochondrial matrix, 3 repeats of an energy transfer sequence, but no ATP-binding motif, suggesting that MFT is an ATP-independent mitochondrial transporter. MFT shares highest homology with a yeast mitochondrial flavin nucleotide transporter and human and rodent carrier proteins involved in ATP/ADP exchange in mitochondria. Northern blot analysis of a human leukemia cell line detected a 1.5-kb transcript and diffuse hybridization between 2.4 and 2.6 kb.