Spermatogenesis Associated Protein 13 (SPATA13)

ASEF2 required the lipid-modified form of CDC42. ABRSH3 also functioned in an autoinhibitory reaction by binding the C-terminal tails of ASEF1 and ASEF2 and inhibiting their GEF activities. Deletion of ABRSH3 or coexpression of the APC armadillo repeat sequence with full-length ASEF2 stimulated filopodia formation in transfected HeLa cells. activation of ASEF1 and ASEF2 involves binding of APC to ABRSH3, which disrupts the autoinhibitory interaction of ABRSH3 with the ASEF C-terminal tail and allows GDP/GTP exchange on CDC42.ASEF2 interacted with neurabin II (PPP1R9B) in human embryonic kidney cells. In response to HGF treatment of HeLa cells, ASEF2, neurabin II, and APC accumulated and colocalized in lamellipodia and membrane ruffles.