Suppressors Of Cytokine Signaling 7 (SOCS7)

SOCS7 recruited NCK into the nucleus. Moreover, depletion of NCK from the cytoplasm triggered dissolution of actin stress fibers and loss of cell polarity.

The longest open reading frame encodes a predicted 485-amino acid protein containing several pro-rich sequences, a putative nuclear localization signal, a pro- and gln-rich region characteristic of nuclear proteins, and an SH2 domain. The third pro-rich region of NAP4 was the only region that bound NCK in the yeast 2-hybrid analysis. Northern blot analysis detected a 4.2-kb NAP4 transcript in fetal brain and fetal lung fibroblasts. An 8.3-kb transcript was also detected in fetal lung fibroblasts. RT-PCR showed NAP4 expression in leukocytes and adult brain.