TP53RK Binding Protein (TPRKB)

The deduced 175-amino acid protein has a calculated molecular mass of 19.7 kD.CGI-121 shares 85% amino acid identity with mouse Cgi-121 and 34% amino acid identity with the yeast homolog. CGI-121-L1 encodes a deduced 214-amino acid protein with a calculated molecular mass of 23.9 kD, and CGI-121-S1 encodes a deduced 142-amino acid protein with a calculated molecular mass of 16.1 kD.Western blot analysis detected a single CGI-121 protein with an apparent molecular mass of 20 kD. Immunofluorescent microscopy detected endogenous CGI-121 distributed in both the nucleus and the cytosol.Neither CGI-121 nor PRPK formed homodimers in vivo or in vitro. Coprecipitation of p53 with PRPK was inhibited by adding recombinant CGI-121 in vitro, suggesting that CGI-121 may inhibit the PRPK-p53 interaction