Tetratricopeptide Repeat Domain Protein 9 (TTC9)

TTC9 encodes a predicted 222-amino acid protein with a calculated molecular mass of 24.4 kD. It has 3 C-terminal tetratricopeptide repeat domains and several potential N-myristoylation sites, but no transmembrane domain. Northern blot analysis detected a 2.5-kb TTC9 transcript in a breast cancer cell line.

Northern blot analysis of several human tissues showed variable expression of 2.5-, 5.1-, and 5.9-kb TTC9 transcripts. Expression of TTC9 was highest in brain, followed by heart. Western blot analysis revealed a 25-kD endogenous TTC9 protein in a breast cancer cell line. Analysis of several normal tissues showed the 25-kD protein in all tissues examined except liver. A doublet of 36 to 37 kD was also expressed in brain.