Tetratricopeptide Repeat Domain Protein 25 (TTC25)

TTC25-GFP is localized to the base of cilia and to ciliary axonemes, and disruption of TTC25 function disrupts ciliogenesis. Mig12-GFP localizes very strongly to the base of cilia and confocal imaging of this construct allows for simple visualization of the planar polarity of basal bodies that underlies polarized ciliary beating. Knockdown of Mig12 disrupts ciliogenesis. Ciliogenesis factors identified in the Xenopus epidermis are required in the midline to facilitate neural tube closure.

The tetratricopeptide repeat (TPR) is a structural motif. It consists in a degenerate 34 amino acid sequence identified in a wide variety of proteins. It is found in tandem arrays of 3–16 motifs, which form scaffolds to mediate protein–protein interactions and often the assembly of multiprotein complexes.