Transcription Factor Yin Yang 2 (YY2)

The cDNA, called YY2, encodes a deduced 372-amino acid protein with several Kruppel-like zinc fingers in the C-terminal region. The YY2 and YY1 proteins share 56.2% overall sequence identity and 86.4% identity in the zinc finger regions. Northern blot analysis of HeLa cell RNA revealed a major YY2 transcript of approximately 7.3 kb and several minor bands. Western blot analysis detected a YY2 protein of about 58 kD and expression of YY2 in all human tissues and cell lines tested except colon.Reporter gene assays demonstrated that YY2 activates transcription of p53 and FOS promoters but at lower rather than higher concentrations in FOS. It also activates MYC and CXCR4 transcription at low concentrations but represses their transcriptional activity at high concentrations.