Transgelin 3 (TAGLN3)

NP25 contains 2 putative EF-hand calcium-binding sites and several putative phosphorylation sites, but no ATP-binding site or glycosylation site. Northern blot analysis detected a 1.35-kb transcript in all brain regions examined. NP25 shares about 97% homology with rat Np25.The deduced 219-amino acid protein contains an EF-hand-like motif, a C-terminal ATP-binding sequence, and several putative phosphorylation sites. Np25 shares significant homology with rat SM22-alpha, Mp20, and calponin. Northern blot analysis detected a single 1.0-kb transcript in rat brain and spinal cord, in most specific rat brain regions examined, and in human cortex and cerebellum. In situ hybridization of rat brain detected highest expression in central amygdaloid nuclei and glomeruli in the granule layer of the cerebellum.