Translin Associated Factor X Interacting Protein 1 (TSNAXIP1)

Using mouse Trax (TSNAX) as bait in a yeast 2-hybrid screen, Bray et al (2002) cloned Tsnaxip1 from a mouse testis cDNA library. The deduced protein contains 709 amino acids and has a coiled-coil structure. Mouse Tsnaxip1 shares 74% homology with the predicted human protein.

Northern blot analysis of mouse tissues detected a 2.8-kb transcript only in testis. Northern blot analysis of Tsnaxip1 in developing germ cells indicated predominantly postmeiotic expression. Fluorescence-tagged Tsnaxip1 transfected into mouse fibroblasts showed a cytoplasmic distribution and concentrated staining around the nucleus.By yeast 2-hybrid analysis and in vitro binding assays, Bray et al. (2002) determined that Tsnaxip1 interacts directly with Trax.