Transmembrane Protein 147 (TMEM147)

TMEM147 interacted with NOMO and nicalin. Knockdown and overexpression studies suggested that nicalin is the key regulator of the complex and that it binds to NOMO prior to inclusion of TMEM147 in the complex. Mutation analysis revealed that the transmembrane domain of nicalin was required for interaction with TMEM147, but not NOMO. TMEM147 contains 7 transmembrane domains. It shares 99% and 78% amino acid identity with mouse and zebrafish Tmem147, respectively. In situ hybridization of zebrafish embryos revealed ubiquitous Tmem147 expression 12 hours after fertilization. Immunohistochemical analysis of HEK293T and HeLa cells revealed colocalization of TMEM147 with NOMO and nicalin in the endoplasmic reticulum.