Tripartite Motif Containing Protein 34 (TRIM34)

TRIM34 contains 2 RING finger domains, 2 RING finger B-box coiled-coil (RBCC) domains, and a C-terminal B30.2 domain. The 488-amino acid medium form has 1 of each of these domains, whereas the 270-amino acid short form has a RING finger domain and a RBCC domain but lacks the B30.2 domain.

Immunoblot analysis detected 98-, 54-, and 31-kD proteins. RT-PCR analysis indicated that each isoform is expressed in placenta and testis. Northern blot analysis revealed expression of a 4-kb transcript predominantly in testis and a 2.4-kb transcript that was highly expressed in placenta, spleen, colon, and blood leukocytes. The long form was expressed only in testis, kidney, and ovary. Expression of the medium form was upregulated in response to alpha-interferon and, later, to gamma-interferon.