TruB Pseudouridine Synthase Homolog 1 (TRUB1)

Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al., 2003).Using database analysis and EST clone assembly, Zucchini et al. (2003) identified TRUB1. The 349-amino acid protein has a predicted molecular mass of 37.3 kD and contains 1 TruB domain. Northern blot analysis showed ubiquitous expression of 5.2-, 3.8-, and 1.75-kb transcripts, with highest expression seen in heart, skeletal muscle, and liver. TRUB1 shares 30% identity and 45% similarity with TRUB2. Phylogenetic analysis showed that TRUB1 and TRUB2 group together with the original eubacterial tRNA synthases, distinct from DKC1 (300126).Zucchini et al. (2003) determined that the TRUB1 gene consists of 8 exons spanning approximately 40 kb.