Trypsin Domain Containing Protein 1 (TYSND1)

All peroxisomal proteins are synthesized in the cytosol, and 2 distinct peroxisomal targeting signals (PTSs), the C-terminal PTS1 and N-terminal PTS2, are used for transport of these proteins into peroxisomes. Proteolytic cleavage of the N-terminal targeting sequence of PTS2 proteins accompanies import into peroxisomes, and many PTS1 proteins undergo C-terminal processing once in the peroxisomal matrix. TYSND1 processes both PTS1 and PTS2 proteins involved in beta-oxidation of fatty acids.Fractionation of rat liver homogenate and Western blot analysis showed that endogenous Tysnd1 was synthesized as a 59-kD protein and converted to 49- and 27-kD forms following import into peroxisomes. Confocal microscopy localized Tysnd1 to peroxisomes of transfected Chinese hamster ovary cells.