Tudor Domain Containing Protein 7 (TDRD7)

TDRD7 contains 1,680 amino acids. RT-PCR ELISA detected highest expression in human adult and fetal brain, with moderate expression in ovary, testis, spleen, lung, and kidney, low expression in pancreas and heart, and no expression in liver and skeletal muscle. All specific brain regions examined showed moderate expression.Endogenous Tdrd7 and PCTAIRE2 localized to the mitochondria in COS-7 cells, and Western blot analysis of rat tissues detected Tdrd7 in brain and testis only. Tdrd7 protein levels in the developing rat brain increased gradually from embryonic day 16 through postnatal day 35 and were correlated with PCTAIRE2 protein levels. Immunohistochemical studies colocalized rat Tdrd7 and PCTAIRE2 protein to the cerebral cortex pyramidal cells and to cerebellar Purkinje cells.