Tumor Suppressor Candidate 2 (TUSC2)

FUS1 is 93% identical to the mouse protein and is probably cytoplasmic. A worm protein, C09e9.1, is approximately 41% identical and contains a bipartite nuclear localization signal. Wide expression of a 1.8-kb transcript that was strongest in heart, lung, skeletal muscle, kidney, and pancreas, followed by brain and liver, with lowest levels in placenta. Moderate expression was detectable in most lung cancer cell lines examined. Mutations were found in approximately 5% of cell lines tested, but none of the genes identified in the search had a high frequency of mutations. Transfection and overexpression of either SUFU or FUS1 overcame the growth-promoting effects of miR378. Expression of miR378 repressed expression of reporter gene constructs harboring the miR378 target sites of SUFU or FUS1.