Tweety Homolog 3 (TTYH3)

The deduced 523-amino acid protein has a calculated molecular mass of 58 kD. TTYH3 has 6 transmembrane domains and a glutamate and aspartate-rich Ca(2+) bowl-like sequence. Northern blot analysis detected a 4.8-kb transcript in brain, heart, skeletal muscle, colon, spleen, kidney, and peripheral blood leukocytes. RT-PCR also found expression in mouse fat, pancreas, thymus, and uterus, and in several mammalian endothelial, kidney epithelial, and neuronal cell lines. Immunohistochemical analysis of mouse tissues detected Ttyh3 in thalamic neurons, heart ventricular cells, skeletal muscle membrane, and renal glomerular mesangium cells. TTYH3 expressed in transfected Chinese hamster ovary (CHO) cells was sensitive to N-glycanase treatment.