UBX Domain Protein 2B (UBXN2B)

Rodent p37 formed a complex with p97 in cytosol and localized to Golgi and endoplasmic reticulum (ER). Small interfering RNA experiments in HeLa cells revealed that p37 was required for Golgi and ER biogenesis. Injection of anti-p37 antibodies into HeLa cells at different stages of the cell cycle showed that p37 was involved in Golgi and ER maintenance during interphase and in their reassembly at the end of mitosis.

In an in vitro Golgi reassembly assay, the p97/p37 complex showed membrane fusion activity that required p115-GM130 (GOLGA2) tethering and SNARE GS15 (BET1L). VCIP135 (VCPIP1) was also required, but its deubiquitinating activity was unnecessary for p97/p37-mediated activities.