Ubiquitin Conjugating Enzyme E2V2 (UBE2V2)

The nucleotide sequence of DDVIT1 is identical to that of the EDPF1 cDNA. Northern blot analysis detected DDVIT1 transcripts in all human tissues and cell lines examined. The authors demonstrated that the 1.4-kb DDVIT1 mRNA was induced in freshly isolated blood monocytes by short-term incubation with vitamin D3. The predicted 145-amino acid DDVIT1 protein does not have hydrophobic regions, and therefore the authors suggested that it is soluble.MMS2 protein formed a specific heteromeric complex with UBC13-encoded E2 and was required for the UBC13-dependent assembly of polyubiquitin chains linked through lysine 63. A ubc13 yeast strain was UV sensitive, and single, double, and triple mutants of the UBC13, MMS2, and ubiquitin genes displayed a similar phenotype.