Ubiquitin Like Modifier Activating Enzyme 1 (UBA1)

UBA1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. The cDNA recognized a single 3.5-kb E1 message that was ubiquitous among tissues and cell lines studied. In vitro translation of the mRNA yielded a major product of approximately 110 kD, which was immunoprecipitated by the antihuman E1 antibody used to identify the clone.Database analysis detected variable UBE1 expression in all human tissues and cell lines examined.