Ubiquitin Specific Peptidase 45 (USP45)

USP45 belongs to the peptidase C19 family.Broadly expressed, with highest levels in ovary, skeletal muscle and spleen. Asp/Asn residue is conserved in all USPs identified herein, with the exceptions of USP30 and USP45 which contain a Ser residue at this position as previously described for the catalytically active USP16, and USP50 which lacks the entire C-terminal region encompassing this third member of the catalytic triad. Interestingly, USP30 and USP45 are active in this assay even though their third catalytic residue is a serine instead of the conserved Asp/Asn. Notably, USP30 and USP45 are also active, despite presenting a serine instead of the conserved Asp/Asn in the catalytic triad, a situation identical to that previously described for USP16 that also possesses a serine residue at the equivalent position.