Unc-45 Homolog B (UNC45B)

The deduced 931-amino acid protein has a calculated molecular mass of 103.4 kD and shares 95% and 56% amino acid identity with mouse Unc45b and human UNC45A, respectively. UNC45B contains an N-terminal triple TPR motif, a unique central region, and a C-terminal UNC45/Cro1/She4p (UCS) domain. EST database analysis showed human UNC45B expression in cDNA libraries derived mostly from heart and tissues containing heart and skeletal muscle. The authors also cloned mouse Unc45b, and Northern blot analysis of mouse tissues detected high expression in skeletal muscle, heart, and whole embryo, with low levels detected in lung. In situ hybridization of mouse embryos detected Unc45b expression in the heart at 8.75 days gestation. Unc45b