Vacuolar Protein Sorting 45 (VPS45)

VPS45A contains a potential N-glycosylation site, an amidation site close to the C terminus, 2 potential N-myristoylation sites, and several consensus phosphorylation sites. Northern blot analysis detected abundant expression in heart, spleen, and testis. Expression was moderate to low in all other tissues examined except lung and liver, in which expression was very low. RT-PCR revealed high VPS45A expression in peripheral blood mononuclear cells and neutrophils, but no expression in human hepatoma and breast cancer cell lines.The deduced 570-amino acid protein has a calculated molecular mass of 65 kD and shares 38% identity with yeast Vps45. Northern blot analysis detected a 2.3-kb transcript in all tissues examined.