YEATS Domain Containing Protein 4 (YEATS4)

GAS41 associated with TIP60 complex core subunits and with H2AZ (H2AFZ), a component of the SWI2/SNF2 (SMARCA2)-related CBP (CREBBP) activator protein (SRCAP) complex, confirming that GAS41 is a genuine subunit of the TIP60 and SRCAP complexes. Either knockdown of GAS41 expression with small interfering RNA or specific interruptions of the C-terminal coiled-coil motif of the GAS41 protein activated the p53 (TP53) tumor suppressor pathway. Activation of the p53 pathway did not result from changes in TIP60 complex assembly or TIP60 coactivator functions for p53. GAS41 was prebound to the promoters of 2 p53 tumor suppressor pathway genes, p21 (CDKN1A) and p14(ARF) (CDKN2A), in normal unstressed cells, but GAS41 dissociated from both promoters in response to p53-activating stress signals.