Yippee Like Protein 3 (YPEL3)

YPEL3 contains 5 exons and spans about 4 kb. Exon 1 is untranslated. The mouse Ypel3 gene has the same structure.The deduced 119-amino acid protein shares 100% amino acid identity with mouse and monkey Ypel3 and 45.5% identity with Drosophila Yippee. YPEL3 shares 83.9 to 89.1% amino acid identity with YPEL1, YPEL2 , and YPEL4 . All YPEL proteins contain an 86-amino acid YPEL consensus sequence. PCR analysis detected YPEL3 expression in all adult and fetal tissues examined. A second YPEL3 transcript was detected in pancreas, placenta, and testis. Immunofluorescent staining of COS-7 green monkey kidney cells localized Ypel3 to the centrosome and nucleolus during interphase and at several punctate structures around the mitotic apparatus during the mitotic phase.