Zinc And Ring Finger Protein 2 (ZNRF2)

ZNRF2 was also widely expressed in nonneural tissues, including mammary gland, testis, colon, and kidney. In situ hybridization of mouse pups and immunohistochemical analysis of adult mouse tissues revealed Znrf2 expression throughout the central nervous system and in sensory neurons of the peripheral nervous system.

Znrf2 was expressed in mature sperm, but not developing spermatogonia, as well as in adipose tissue, columnar epithelia of the gut, and hair follicles. Immunohistochemical analysis and fractionation of adult mouse brain localized Znrf1 and Znrf2 to the presynaptic region, with Znrf1 on synaptic vesicles and Znrf2 on presynaptic plasma membranes. In mouse fibroblasts, fluorescence-tagged Znrf2 colocalized with an endosome/lysosome marker.