Zinc Finger And BTB Domain Containing Protein 4 (ZBTB4)

The deduced 1,013-amino acid ZBTB4 protein contains an N-terminal BTB/PPZ domain followed by 4 zinc fingers, a proline-rich domain, a glutamate-rich domain, 2 additional zinc fingers, and an additional C-terminal proline-rich region. The BTB/POZ domain of ZBTB4 is interrupted by a 60-amino acid stretch, and the N-terminal zinc finger region of ZBTB4 contains the KAISO-like triple zinc finger domain. Northern blot analysis detected Zbtb4 in all mouse tissues examined, with highest expression in brain, lung, kidney, muscle, and heart. In situ hybridization of mouse brain detected Zbtb4 mainly in neurons. Expression was highest in hippocampus, in structures involved in olfaction, in arcuate nuclei, in motor nuclei of the brainstem, and in granular layer of the cerebellum.