Zinc Finger And BTB Domain Containing Protein 6 (ZBTB6)

Sequence analysis predicted that the 424-amino acid protein contains 4 C-terminal zinc finger motifs and an N-terminal domain of 120 amino acids found in many different zinc finger proteins and a group of non-zinc finger poxvirus proteins. RNase protection analysis indicated wide expression of ZID, with highest levels in brain. Binding analysis showed interaction of the zinc fingers with specific oligonucleotides, and these interactions could be inhibited by the POZ domain. This inhibitory effect did not result from a specific interaction between the POZ domain and the zinc fingers. Immunoprecipitation analysis suggested that the POZ domain is a protein-protein interaction domain. Immunofluorescence microscopy demonstrated that the nuclear localization of ZID is dependent on the presence of the POZ domain.