Zinc Finger Protein 428 (ZNF428)

ZNF420, Contains 1 C2H2-type zinc finger. A zinc finger protein is a DNA-binding protein domain consisting of zinc fingers ranging from two in the Drosophila regulator ADR1, the more common three in mammalian Sp1 up to nine in TFIIIA. They occur in nature as the part of transcription factors conferring DNA sequence specificity as the DNA-binding domain. They have also found use in protein engineering due to their modularity and have prospects as components of tools for use in therapeutic gene modulation and zinc finger nucleases. It consists of 2 beta strands, one alpha helix and a hairpin structure. The first Zn binds to Cys-3 of 1st beta strand, second Zn binds to Cys-6 that is present in the tight loop that separates the two beta strands. The other two Zn is coordinated to Cys 19 & 23 which are present in C-terminal half of the alpha helix.