Recombinant Cluster Of Differentiation 73 (CD73) Homo sapiens (Human) Recombinant protein

NT5E; NT5-E; E5NT; E5-NT; NTE; EN; ENT; 5'-Nucleotidase, Ecto

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Molecular Mass 53.6kDa, Accurate 53kDa(Analysis of differences refer to the manual)
  • Residues & TagsLeu29~Thr500 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.4
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  • Recombinant Cluster Of Differentiation 73 (CD73) Packages (Simulation)
  • Recombinant Cluster Of Differentiation 73 (CD73) Packages (Simulation)
  • Recombinant Cluster Of Differentiation 73 (CD73) Gene sequencing
  • RPB250Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammationPubmed:25640206
  • Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment …10.1016:j.talanta.2018.01.044
  • Expression and clinical significance of serum NT5E protein in patients with colorectal cancerPubmed: 30932882

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