Active Catalase (CAT) Rattus norvegicus (Rat) Active protein

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point7.5
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  • Active Catalase (CAT) Packages (Simulation)
  • Active Catalase (CAT) Packages (Simulation)
  • APC418Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Catalase (CAT) is an antioxidant enzyme present in all aerobic organisms. It is known to catalyze H2O2 into water and oxygen in an energy-efficient manner in the cells exposed to environmental stress. H2O2 will have specific absorbance at 240 nm . when we add CAT， the absorbance wil decrease, thus the activiy of CAT can be measured by caculating H2O2 absorbance decrease. The reaction was performed in adding 10ul (dilute with 50mM Potassium Phosphate Buffer, pH 7.0) recombinant Rat CAT to 290ul substrate mixture solution (50mM Potassium Phosphate Buffer, pH 7.0, 0.036% H2O2, allow the substrate to equilibrate to 25 °C), quickly mixed, then record the time required for the A240 to decrease from 0.45 to 0.40 absorbance units. One unit of catalase will decompose 1.0 µmole of H2O2 per minute at pH 7.0 at 25 °C. The activity of recombiant Rat CAT is 176000 U/mg.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic AneurysmAhajournals: source
  • Phloroglucinol protects gastric mucosa against ethanol-induced injury through regulating myeloperoxidase and catalase activitiesPubMed: 20880383
  • Antioxidant profile of salivary glands in high fat diet-induced insulin resistance ratsPubmed: 24106991
  • Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients.Pubmed: 24142649
  • Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar ratsSpringer: Source
  • 过氧化氢酶基因 ３８９Ｃ＞Ｔ多态位点在重庆汉族人群中的分布及其与噪音性耳聋的 关联研究Tmmu:Source
  • Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, PubMed: 26674569
  • Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetesPubMed: 26143097
  • Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human salivaPubMed: 26608886
  • КЛИНИЧЕСКОЕ ЗНАЧЕНИЕ ФАКТОРОВ КЛЕТОЧНОЙ РЕГУЛЯЦИИ И ФЕРМЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ У ПАЦИЕНТОК РЕПРОДУКТИВНОГО ВОЗРАСТА С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИdocs:225188
  • Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients.pubmed:27748187
  • Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokinesjcrt:5
  • Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects.pubmed:28242484
  • Laminarin protects against hydrogen peroxide-induced oxidative damage in MRC-5 cells possibly via regulating NRF2pubmed:28785522
  • Evaluation of ADMA, carbonyl groups, CAT and NKA in depressed patients with and without posttraumatic stress disorder.pubmed:28554099
  • Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oilPubmed:29660615
  • Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snails10.1080:10807039.2018.1460800
  • Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathwayPubmed:30116359
  • A red pumpkin seed extract reduces melanosome transfer to keratinocytes by activation of Nrf2 signalingPubmed:30047218
  • Cholecystectomy Patients with High Plasma Level of Catalase Have Significantly Lower Analgesia Requirement: A Prospective Study of Two Different …Pubmed: 30194197
  • Plasma Catalase in Relation to Pain Following Midline Laparotomy: A Prospective Study of Patients with Benign Diseases and Patients with CancerPubmed: 30396975
  • Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed ratsDoi: 10.1016/j.joim.2018.12.002
  • Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formationPubmed: 30606939
  • An in vitro protocol to study the effect of hyperglycemia on intracellular redox signaling in human retinal pigment epithelial (ARPE-19) cellsPubmed: 30628023
  • Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer PatientsPubmed: 30711961
  • Rectus Sheath Block (RSB) Analgesia Could Enhance Significantly the Patient Satisfaction Following Midline Laparotomy in Benign Disease and in Cancer: A …Pubmed: 30842172
  • MicroRNA‑21 contributes to the puerarin‑induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injuryPubmed: 31115556
  • Selected elements of extracellular matrix of the skin in diabetes and insulin resistancePubmed: 31146169
  • European Journal of Biomedical AND Pharmaceutical sciences
  • The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and CancerPubmed: 31892574
  • A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 SignalingPubmed: 32135248
  • Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease DevelopmentPubmed: 32751983
  • AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS
  • In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells
  • Lutein reverses hyperglycemia-mediated blockage of Nrf2 translocation by modulating the activation of intracellular protein kinases in retinal pigment epithelial (ARPE …
  • Plasma catalase (CAT) levels in gallstone disease: Cholecystectomy patients with high plasma levels of CAT have significantly lower number of analgesic …
  • Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring
  • Toxic Trace Metals and Pathological Changes in Organs of Rats Fed with Extract of Polluted Grasses
  • Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ¡­33689849
  • Mechanisms of Trx2/ASK1-Mediated Mitochondrial Injury in Pemphigus Vulgaris33763469
  • Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡­34064937
  • Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats34278476
  • Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis34945751
  • Plasma Concentration of the Lipid Peroxidation (LP) Biomarker 4-Ηydroxynonenal (4-HNE) in Benign and Cancer PatientsPubmed:35241533
  • α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat dietPubmed:35569738
  • Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory …Pubmed:35204215

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