Active Collagen Type I Alpha 1 (COL1a1) Homo sapiens (Human) Active protein

COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point5.0
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  • Active Collagen Type I Alpha 1 (COL1a1) Packages (Simulation)
  • Active Collagen Type I Alpha 1 (COL1a1) Packages (Simulation)
  • APA350Hu61.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Type I collagen is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. It is a heterotrimer that consists of two alpha 1 chains and one alpha 2 chain, although homotrimers consisting of three identical alpha 1 chains have also been described. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human COL1a1 and recombinant human COL1a2. Briefly, biotin-linked COL1a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to COL1a2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of COL1a1 and COL1a2 was shown in Figure 1, the EC50 for this effect is 10.13 ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Simvastatin-loaded porous implant surfaces stimulate preosteoblasts differentiation: an in vitro studyScienceDirect: S1079210410004919
  • Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro studyWiley: source
  • Age effects on extracellular matrix production of vocal fold scar fibroblasts in ratsPubmed: 24077847
  • Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study Pubmed: 24495429
  • Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells.Pubmed: 24370188
  • Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propriaPubmed:25545625
  • Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-cultureUnivmed:Source
  • The vitamin D receptor agonist, calcipotriol, modulates fibrogenic pathways mitigating liver fibrosis in-vivo: An experimental study.pubmed:27477355
  • Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396
  • Prevention of Diabetic Nephropathy by Modified Acidic Fibroblast Growth Factorpubmed:28768285
  • Effect of TGFβ1, TGFβ3 and keratinocyte conditioned media on functional characteristics of dermal fibroblasts derived from reparative (Balb/c) and …Pubmed:29637306
  • LOXL2, a copper-dependent monoamine oxidase, activates lung fibroblasts through the TGF-β/Smad pathwayPubmed: 30320382
  • Association between Plasma HMGB-1 and Silicosis: A Case-Control StudyPubmed: 30558126
  • Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detectionPubmed: 31706945
  • Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm
  • Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response
  • Inhibition of eEF2K synergizes with glutaminase inhibitors or 4EBP1 depletion to suppress growth of triple-negative breast cancer cells33911160
  • Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro ModelPubmed:35682677

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