Active Complement Factor B (CFB) Mus musculus (Mouse) Active protein

CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point5.1
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  • Active Complement Factor B (CFB) Packages (Simulation)
  • Active Complement Factor B (CFB) Packages (Simulation)
  • APC011Mu03.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Complement factor B (CFB), a 95-kDa protein, is a crucial catalytic element of the alternative pathway (AP) of complement. After binding of CFB to C3b, activation of the AP depends on the proteolytic cleavage of CFB by factor D to generate the C3 convertase (C3bBb). The C3 convertase contains the catalytic subunit of CFB (Bb), the enzymatic site for the cleavage of a new molecule of C3 into C3b. Thus, a binding ELISA assay was conducted to detect the association of recombinant mouse CFB with recombinant mouse CFD. Briefly, biotin-linked recombinant mouse CFB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CFD-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse CFB and recombinant mouse CFD was shown in Figure 1, the EC50 for this effect is 1.238 ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Complement in patients receiving maintenance hemodialysis: functional screening and quantitative analysisBioMed: 14712369
  • Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MSACS: pr300330z
  • Atypical hemolytic uremic syndrome: Korean pediatric seriesPubMed: 25443527
  • Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitisPubmed:27235358
  • Adipose tissue complement factor B promotes adipocyte maturation.pubmed:29137982
  • Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection
  • Identification of susceptibility locus shared by IgA nephropathy and inflammatory bowel disease in a Chinese Han populationPubmed: 31857673
  • Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis33367879
  • Changes in complement alternative pathway components, factor B and factor H during dengue virus infection in the AG129 mouse33410734
  • Early Diagnostic Ability of Human Complement Factor B in Pancreatic Cancer Is Partly Linked to Its Potential Tumor-Promoting Role34766501
  • A Three-Protein Panel to Support the Diagnosis of Sepsis in ChildrenPubmed:35329889

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