Active Hepatocyte Growth Factor (HGF) Homo sapiens (Human) Active protein

HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point9.0
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  • Active Hepatocyte Growth Factor (HGF) Packages (Simulation)
  • Active Hepatocyte Growth Factor (HGF) Packages (Simulation)
  • APA047Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Hepatocyte growth factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells and T cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration, and in wound healing. Besides, Matrix Metalloproteinase 9 (MMP9) has been identified as an interactor of HGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HGF and recombinant mouse MMP9. Briefly, HGF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to MMP9 -coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti- HGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human HGF and recombinant mouse MMP9 was shown in Figure 1, the EC50 for this effect is 0.35 ug/mL.

Usage

Reconstitute in ddH2O to a concentration less than or equal to 0.1mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heartLWW: 01000
  • Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinomaBioMed: 14795876
  • Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver FailureSpringerLink: k22823l138x2344x
  • Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic ChildrenPubMed: PMC3694825
  • Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)PubMed: PMC3762604
  • BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopausePubMed: PMC3640998
  • Effect of Mesenchymal Stem Cells on Doxorubicin-Induced FibrosisPubMed: PMC3584430
  • Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver CirrhosisPubMed: 26448742
  • Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaPubMed: 26695151
  • Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injuryPubMed: 25863963
  • Silymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated ratsPubmed:27261582
  • Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosisPubmed:27186801
  • Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cellsPubmed:27323064
  • Changes in HGF levels after a progressive test before and after Tabata HIIT programprofile:Pawel_Wolanski3
  • Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats10.1186/s13287-017-0739-3
  • Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c‑Met/PI3K/AKT in vitro10.3892/ol.2017.7301
  • Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure.pubmed:28679425
  • Pulmonary pericytes regulate lung morphogenesisPubmed:29934496
  • Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke …Pubmed:29650950
  • Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitroPubmed:29375714
  • Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in RatsPubmed:29796817
  • Inherent control of hepatocyte proliferationInherent control of hepatocyte proliferation after subtotal liver resectionPubmed: 31297922
  • What is the Best Degree of Hyaluronic Acid Crosslinking in Increasing Growth Factors Level of Platelet-Rich Fibrin Lysate?Pubmed: 31239605
  • Efficacy of autologous bone marrow mononuclear cell transplantation in dogs with chronic spinal cord injuryPubmed: 32821665
  • Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care33469783
  • Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377

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