Active Klotho (KL) Rattus norvegicus (Rat) Active protein

KLa; Klotho Alpha

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.4
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  • Active Klotho (KL) Packages (Simulation)
  • Active Klotho (KL) Packages (Simulation)
  • Active Klotho (KL) Figure. Gene Sequencing (Extract)
  • APH757Ra01.png Figure. SDS-PAGE
  • Active Klotho (KL) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Klotho (KL) is a transmembrane protein that, in addition to other effects, provides some control over the sensitivity of the organism to insulin and appears to be involved in aging. The Klotho protein is a novel β-glucuronidase capable of hydrolyzing steroid β-glucuronides. Genetic variants in KLOTHO have been associated with human aging, and Klotho protein has been shown to be a circulating factor detectable in serum that declines with age. The binding of certain fibroblast growth factors (FGF's) viz., FGF19, FGF20, and FGF23, to their fibroblast growth factor receptors, is promoted via their interactions as co-receptors with β-Klotho. Besides, Fibroblast Growth Factor 23 (FGF23) has been identified as an interactor of KL, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat KL and recombinant rat FGF23. Briefly, KL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to FGF23-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-KL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of KL and FGF23 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of KL with FGF23.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculaturePubmed: 24012609
  • Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in MiceCom:Source
  • α-Klotho is an acute phase protein and altered by restraint stress in micePubmed:Pmc4203207
  • Les biomarqueurs des calcifications vasculairesPubMed: 26069069
  • Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with KlothoPubMed: 25873020
  • Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3PubMed: 25899975
  • 抗衰老蛋白Klotho 阻断高糖培养的大鼠肾小球系膜细胞TLR4/NF-κB p65/NGAL 通路的激活及其作用机制Cn: Article
  • The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanismPubMed: 26419931
  • Soluble Klotho protein as a novel serum biomarker in patients with acromegalypmc:PMC4754356
  • Klotho Deficiency Aggravates Tacrolimus-Induced Renal Injury via the Phosphatidylinositol 3-Kinase-Akt-Forkhead Box Protein O PathwayPubmed:27174564
  • Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice.pubmed:28387374
  • Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management?pubmed:28429560
  • Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patientsissn:2394-2916
  • Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in miceISSN:1936-2625
  • Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat modelpubmed:28943597
  • Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion InjuryPubmed:29550818
  • Advanced maternal age impairs spatial learning capacity in young adult mouse offspringPubmed:29636887
  • Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complicationsPubmed:30042059
  • Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal …Pubmed:30004089
  • High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney diseasePubmed: 30189026
  • The role of klotho in chronic kidney diseasePubmed: 30348110
  • Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regenerationPubmed: 30451844
  • Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndromePubmed: 30770217
  • The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic ratsPubmed: 30801205
  • CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protectionPubmed: 33223223
  • Effects of salt intervention on serum levels of Klotho influenced by salt sensitivityPubmed: 33164306
  • Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic ratsPubmed: 32744464
  • Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic ratsPubmed: 32438076
  • P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy.Pubmed: 32377919
  • Ameliorating Effect of Klotho Protein on Rat Heart during I/R InjuryPubmed: 33123314
  • Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With SchizophreniaPubmed: 32612508
  • ¦ÁKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy33675964
  • Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma CellsPubmed:35112226
  • Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance
  • Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome

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