Active Lactoferrin (LTF) Homo sapiens (Human) Active protein

HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Pointn/a
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  • Active Lactoferrin (LTF) Packages (Simulation)
  • Active Lactoferrin (LTF) Packages (Simulation)
  • APA780Hu01.png Figure. SDS-PAGE
  • Active Lactoferrin (LTF) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. In particular, lactoferrin provides antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands. LTF is known to activate immune cells to produce several cytokines, such as tumor necrosis factor TNF-a, thus, a stimulation assay was conducted to detect the activity of LTF using human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into wells of 24-well plates at a density of 5×106 cells/mL in RPMI-1640 with the addition of various concentrations of recombinant human LTF. After incubation for 20 hours, the concentration of TNF-a in the cell supernatant was detected using an ELISA kit. TNF-a levels in the cell supernatant of spleen cells increased significantly after stimulated with LTF, the data was shown in table 1.

Figure. TNF-a concentration in the cell supernatant of THP-1 cells up-regulated by LTF.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Changes of hepatic lactoferrin gene expression in two mouse models of the acute phase reactionScienceDirect: S1357272511002524
  • Changes in composition of colostrum of Egyptian buffaloes and Holstein cowsBioMed: 17466148
  • p38MAPK, ERK and PI3K Signaling Pathways Are Involved in C5a-Primed Neutrophils for ANCA-Mediated Activation PlosOne: source
  • Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during PregnancyPubmed:25505239
  • Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4Pubmed:26974438
  • Salivary lactoferrin is transferred into the brain via the sublingual routepubmed:28351211
  • Effects of fluticasone propionate and budesonide on the expression of immune defense genes in bronchial epithelial cellsPubmed:29627483
  • TLR2 agonist Pam3CSK4 enhances the antibacterial functions of GM-CSF induced neutrophils to methicillin-resistant Staphylococcus aureus
  • Assessment of the Diagnostic Value of IL-10 and Lactoferrin by BIRADS Categories in Breast Neoplasms
  • A Three-Protein Panel to Support the Diagnosis of Sepsis in ChildrenPubmed:35329889
  • Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein ComponentsPubmed:35438093
  • Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung InjuryPubmed:35625931

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