Active Myostatin (MSTN) Homo sapiens (Human) Active protein

GDF8; Growth Differentiation Factor 8

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Overview
Properties
  • Buffer FormulationPBS, pH7.4.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point6.8
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  • Active Myostatin (MSTN) Packages (Simulation)
  • Active Myostatin (MSTN) Packages (Simulation)
  • APB653Hu01.jpg SDS-PAGE
  • Active Myostatin (MSTN) Figure. Western Blot; Sample: Recombinant MSTN, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Myostatin (MSTN) also known as growth differentiation factor 8(GDF-8) is a member of the TGF beta protein family. Myostatin is aa secreted growth differentiation factor that produced and released by myocytes. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Besides, Bone Morphogenetic Protein 1 (BMP1) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MSTN and recombinant human BMP1. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of MSTN and BMP1 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized RatsSapub:Source
  • Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in micePubMed: 26539241
  • Tumor inoculation site affects the development of cancer cachexia and muscle wastingPubMed: 26016447
  • Induction of transient tenogenic phenotype of high density cultured human dermal fibroblastsPubMed: 25748814
  • Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary DiseasePubmed:26998756
  • The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized RatsPubmed:27272029
  • Irisin and Myostatin Levels in Patients with Graves' Disease.pubmed:27986127
  • Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Ratspubmed:29242963
  • Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis.pubmed:28740214
  • Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigsPubmed: 30203098
  • Predictive value of serum myostatin for the severity and clinical outcome of heart failurePubmed: 31056368
  • Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar ratsPubmed: 31960436
  • Myostatin and muscle atrophy during chronic kidney diseasePubmed: 32974666
  • Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance …34868459

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