Active Toll Like Receptor 2 (TLR2) Homo sapiens (Human) Active protein

CD282; TIL4; Toll/interleukin-1 receptor-like protein 4

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point5.8
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  • Active Toll Like Receptor 2 (TLR2) Packages (Simulation)
  • Active Toll Like Receptor 2 (TLR2) Packages (Simulation)
  • APA663Hu02.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

TLR2 is a member of TLR family which is type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid precursor that contains a signal sequence (aa 1-18), an extracellular domain (aa 19-588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells endothelial cells, and hepatocytes. TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6, and possibly other co-receptors. These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers bind triacylated lipopeptides, while the TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides. A functional binding ELISA assay was conducted to detect the interaction of recombinant human TLR2 and recombinant human TLR1. Briefly, TLR2 were diluted serially in PBS, with 0.01% BSA (pH7.4). Duplicate samples of 100 μl were then transferred to TLR1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TLR2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450 nm immediately. The binding activity of TLR2 and TLR1 was shown in Figure 1, and this effect was in a dose dependent manner, the EC50 was 0.21 ug/ml.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Expression of soluble Toll-like receptors in pleural effusionsCMJ: 2010823483999506297
  • Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway InflammationPubMed: PMC3639905
  • TLR2 in Pleural Fluid Is Modulated by Talc Particles during PleurodesisPubMed: PMC3529880
  • The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore MutationPubmed: 24187552
  • Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranesPubmed:25028801
  • Cytokines and Toll-Like Receptors in the Immune Response to Influenza VaccinationPubmed:25248345
  • Correlation?between?salivary?Toll like receptor-2?concentration?and early childhood caries.Pubmed:25265998
  • Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapyPubmed:25266302
  • The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseasesPubmed:25406630
  • Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in micePubMed: 25834936
  • Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage.Pubmed:27223696
  • The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-PatientsPubmed:26835476
  • Pentraxin 3 and other inflammatory biomarkers related to atrial fibrillation in cardiac surgerypubmed:27852958
  • Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions.pubmed:27882497
  • Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic jointspubmed:28093922
  • Features of the Pattern Recognition Receptor TLR-2 Expression and Cytokines IL-2, IL-4 Profile in Children with Food Hypersensitivity10.12988:bmgt.2018.812
  • Prospective study on the significance of mucosal barrier dysfunction markers in assessing the effectiveness of the new strategy for treating food hypersensitivity in …10.22141:2224-0551.13.2.2018.129548
  • Vinpocetine regulates levels of circulating TLRs in Parkinson's disease patientsPubmed: 30315378
  • PROGNOSTIC ROLE OF IL-4, IL-2 AND TLR-2 FOR PREDICTING OF COURSE OF FOOD ALLERGY IN CHILDRENDoi: 10.29256/v.02.02.2018.escbm71
  • STUDY OF THE ACTION OF CHROMIUM-CONTAINING BIOPREPARATIONS FROM SPIRULINA ON INSULIN ACTIVITY IN RATSDoi: 10.29256/v.02.02.2018.escbm72
  • Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4
  • Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apneaPubmed: 32069296
  • ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE …Pubmed: 32242853
  • ДОбОвА вАРІАбЕЛЬНІСТЬ РІвНЯ РОзЧИННИХ ТОЛ-пОДІбНИХ РЕцЕпТОРІв 2 в СИРОвАТцІ КРОвІ ЖІНОК, ХвОРИХ НА РЕвМАТОїДНИй АРТРИТ: зв' …
  • FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE …
  • Levels of toll-like receptors 2 and 4 in serum of men with postinfarction cardiosclerosis and its relationship with antiphospholipid syndrome components
  • A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis34244993
  • Altered innate immune profile in blood of systemic mastocytosis patientsPubmed:35734269

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