Active Toll Like Receptor 2 (TLR2) Homo sapiens (Human) Active protein

CD282; TIL4; Toll/interleukin-1 receptor-like protein 4

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point4.6
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  • Active Toll Like Receptor 2 (TLR2) Packages (Simulation)
  • Active Toll Like Receptor 2 (TLR2) Packages (Simulation)
  • APA663Hu04.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

TLR2 is a member of TLR family which is type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid precursor that contains a signal sequence (aa 1-18), an extracellular domain (aa 19-588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells, endothelial cells and hepatocytes. TLR2 stimulation induces mTORC1 activation through TIRAP, which is essential for TLR2-mediated IFN-γ production. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TLR2 and recombinant human TIRAP. Briefly, TLR2 were diluted serially in PBS, with 0.01% BSA (pH7.4). Duplicate samples of 100 ul were then transferred to TIRAP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TLR2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of TLR2 and TIRAP was shown in Figure 1, and this effect was in a dose dependent manner, the EC50 was 0.013 ug/ml.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Expression of soluble Toll-like receptors in pleural effusionsCMJ: 2010823483999506297
  • Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway InflammationPubMed: PMC3639905
  • TLR2 in Pleural Fluid Is Modulated by Talc Particles during PleurodesisPubMed: PMC3529880
  • The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore MutationPubmed: 24187552
  • Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranesPubmed:25028801
  • Cytokines and Toll-Like Receptors in the Immune Response to Influenza VaccinationPubmed:25248345
  • Correlation?between?salivary?Toll like receptor-2?concentration?and early childhood caries.Pubmed:25265998
  • Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapyPubmed:25266302
  • The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseasesPubmed:25406630
  • Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in micePubMed: 25834936
  • Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage.Pubmed:27223696
  • The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-PatientsPubmed:26835476
  • Pentraxin 3 and other inflammatory biomarkers related to atrial fibrillation in cardiac surgerypubmed:27852958
  • Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions.pubmed:27882497
  • Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic jointspubmed:28093922
  • Features of the Pattern Recognition Receptor TLR-2 Expression and Cytokines IL-2, IL-4 Profile in Children with Food Hypersensitivity10.12988:bmgt.2018.812
  • Prospective study on the significance of mucosal barrier dysfunction markers in assessing the effectiveness of the new strategy for treating food hypersensitivity in …10.22141:2224-0551.13.2.2018.129548
  • Vinpocetine regulates levels of circulating TLRs in Parkinson's disease patientsPubmed: 30315378
  • PROGNOSTIC ROLE OF IL-4, IL-2 AND TLR-2 FOR PREDICTING OF COURSE OF FOOD ALLERGY IN CHILDRENDoi: 10.29256/v.02.02.2018.escbm71
  • STUDY OF THE ACTION OF CHROMIUM-CONTAINING BIOPREPARATIONS FROM SPIRULINA ON INSULIN ACTIVITY IN RATSDoi: 10.29256/v.02.02.2018.escbm72
  • Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4
  • Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apneaPubmed: 32069296
  • ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE …Pubmed: 32242853
  • ДОбОвА вАРІАбЕЛЬНІСТЬ РІвНЯ РОзЧИННИХ ТОЛ-пОДІбНИХ РЕцЕпТОРІв 2 в СИРОвАТцІ КРОвІ ЖІНОК, ХвОРИХ НА РЕвМАТОїДНИй АРТРИТ: зв' …
  • FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE …
  • Levels of toll-like receptors 2 and 4 in serum of men with postinfarction cardiosclerosis and its relationship with antiphospholipid syndrome components
  • A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis34244993
  • Altered innate immune profile in blood of systemic mastocytosis patientsPubmed:35734269

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