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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Recombinant Amelogenin, X-Linked (AMELX)
AMGX; AIH1; ALGN; AMG; AMGL; Amelogenesis Imperfecta 1
Organism species Homo sapiens (Human)
Product No.RPJ645Hu01
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass17.3kDa
Predicted isoelectric point6.8
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
Subcellular Location Secreted, extracellular space, extracellular matrix
ResiduesTyr49~Arg187 (Accession # Q99217) with N-terminal His-Tag
FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- YP SYGYEPMGGW LHHQIIPVLS QQHPPTHTLQ PHHHIPVVPA QQPVIPQQPM MPVPGQHSMT PIQHHQPNLP PPAQQPYQPQ PVQPQPHQPM QPQPPVHPMQ PLPPQPPLPP MFPMQPLPPM LPDLTLEAWP STDKTKR
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAJ645Hu21Monoclonal Antibody to Amelogenin, X-Linked (AMELX)WB, ICC, IHC-P, IHC-F, ELISA
PAJ645Hu01Polyclonal Antibody to Amelogenin, X-Linked (AMELX)WB, ICC, IHC-P, IHC-F, ELISA
RPJ645Hu01Recombinant Amelogenin, X-Linked (AMELX)SDS-PAGE; WB; ELISA; IP.
SEJ645HuELISA Kit for Amelogenin, X-Linked (AMELX)ELISA