Recombinant Cystatin B (CSTB) Rattus norvegicus (Rat) Recombinant protein

Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationCytoplasm
  • Molecular Mass 12.3kDa, Accurate 15kDa(Analysis of differences refer to the manual)
  • Residues & TagsMet2~Phe98 (Accession # P01041) with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point6.5
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  • Recombinant Cystatin B (CSTB) Packages (Simulation)
  • Recombinant Cystatin B (CSTB) Packages (Simulation)
  • RPJ322Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disordersPubMed: 23291538
  • Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancerPubMed: PMC3754495
  • Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal ApoptosisPubMed: PMC3365072
  • Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophagesPubmed:25209871
  • A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive systemScience: Article
  • Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis.pubmed:28766361

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