Eukaryotic Prekallikrein (PK) Rattus norvegicus (Rat) Eukaryotic protein

KLKB1; KLK3; Fletcher Factor; Kallikrein B,Plasma; Kininogenin; Plasma prekallikrein

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Overview
Properties
  • Source Eukaryotic expression, Host 293F cell
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 70.7kDa, Accurate 75kDa(Analysis of differences refer to the manual)
  • Residues & TagsGly20~Ala638 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point8.1
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  • Eukaryotic Prekallikrein (PK) Packages (Simulation)
  • Eukaryotic Prekallikrein (PK) Packages (Simulation)
  • EPB801Ra61.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Altered activities of kininase II, an angiotensin converting enzyme, prekallikrein, and nitric oxide in Kuwaiti patients with type 2 diabetesPubMed: 25964383
  • Factor XII–mediated contact activation related to poor prognosis in disseminated intravascular coagulationPubMed: 26706311
  • Factor XII-mediated contact activation related to poor prognosis in disseminated intravascular coaCavia (Guinea pig )lationPubmed:26706311
  • The First Case of Congenital Prekallikrein Deficiency in Korea With a Novel Pathogenic Variant (c. 1198G> T)Pubmed: 30430790
  • Huaier shows anti©\cancer activities by inhibition of cell growth, migration and energy metabolism in lung cancer through PI3K/AKT/HIF©\1¦Á pathway33377619
  • Contact system activation in disseminated intravascular coagulation: activities of prekallikrein and high-molecular-weight kininogen are significant risk factorsPubmed:34993714

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