Recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1)Homo sapiens (Human)Recombinant protein

PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase

  • Product No.RPH839Hu01
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationCytoplasm
  • Predicted Molecular Mass30.6kDa
  • Accurate Molecular Mass30kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla74~Ile333 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsFreeze-dried powder
  • Purity> 97%
  • Isoelectric Point7.2
  • ApplicationsSDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
  • DownloadInstruction Manual
  • UOM10ug50ug200ug1mg1g
  • FOBUS$ 170US$ 424US$ 848US$ 2544US$ 63600
  • Recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1)Package and Components
  • Recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1)Package and Components
    • RPH839Hu01.jpgSDS-PAGE
    • CertificateISO9001: 2008, ISO13485: 2003 Registered

    SEQUENCE

    USAGE

    Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

    STORAGE

    Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

    STABILITY

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Reference

    • Molecular & Cellular ProteomicsIdentification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic AnalysisMcponline: 008821
    • Molecular & Cellular ProteomicsIdentification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic AnalysisPubmed: 22064513
    • Clinical Cancer ResearchIsocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancerPubmed: 24046070
    • Onco Targets TherThe effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cellsPubMed: 25914546