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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1)
Organism species Homo sapiens (Human)
Product No.RPH839Hu01
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass30.8kDa
Predicted isoelectric point7.2
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
Subcellular Location Cytoplasm. Peroxisome
ResiduesAla74~Ile333 (Accession # O75874) with N-terminal His-Tag
FormulationSupplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF- ATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASI
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAH839Hu21Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1)WB, ICC, IHC-P, IHC-F, ELISA
PAH839Hu01Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1)WB, ICC, IHC-P, IHC-F, ELISA
RPH839Hu01Recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1)SDS-PAGE; WB; ELISA; IP.
SCH839HuCLIA Kit for Isocitrate Dehydrogenase 1, Soluble (IDH1)CLIA
SEH839HuELISA Kit for Isocitrate Dehydrogenase 1, Soluble (IDH1)ELISA