Recombinant Lipoprotein lipase (LPL) Rattus norvegicus (Rat) Recombinant protein

LIPD; Lipase, Lipoprotein

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Secreted
  • Molecular Mass 53.9kDa, Accurate 54kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla28~Gly474 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point8.2
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  • Recombinant Lipoprotein lipase (LPL) Packages (Simulation)
  • Recombinant Lipoprotein lipase (LPL) Packages (Simulation)
  • Recombinant Lipoprotein lipase (LPL) Figure . Gene Sequencing (extract)
  • RPA386Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Severity of Diabetes Governs Vascular Lipoprotein Lipase by Affecting Enzyme Dimerization and DisassemblyPubMed: 21646389
  • Clinical efficacy of serum lipase subtype analy-sis for the differential diagnosis of pancreatic and non-pancreatic lipase elevationPubmed:27243230
  • ReCavia (Guinea pig )lation of Plasma Lipid Homeostasis by Hepatic Lipoprotein Lipase in Adult MicePubmed:27234787
  • Polysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet10.1016/j.jff.2017.07.020
  • Mass Spectrometric Evaluation of Upstream and Downstream Process Influences on Host Cell Protein Patterns in Biopharmaceutical ProductsPubmed: 30767403
  • Integrin β3 Deficiency Results in Hypertriglyceridemia Via Disrupting LPL (Lipoprotein Lipase) SecretionPubmed: 32237906
  • Abdominal fat distribution modulates the metabolic effects of exogenous ketones in individuals with new-onset prediabetes after acute pancreatitis: Results from a randomized placebo-controlled trial34024503
  • Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory …Pubmed:35204215

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