Recombinant Permeability Glycoprotein (Pgp) Homo sapiens (Human) Recombinant protein

CD243; ABCB1; ABC20; CLCS; GP170; MDR1; PGY1; P-Glycoprotein; Multidrug resistance protein 1; ATP-Binding Cassette,Sub-Family B(MDR/TAP),Member 1; Colchicin Sensitivity

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Molecular Mass 57.9kDa, Accurate 60kDa(Analysis of differences refer to the manual)
  • Residues & TagsPhe394~Val672 with N-terminal His and GST Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 80%
  • Isoelectric Point7.0
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  • Recombinant Permeability Glycoprotein (Pgp) Packages (Simulation)
  • Recombinant Permeability Glycoprotein (Pgp) Packages (Simulation)
  • Recombinant Permeability Glycoprotein (Pgp) Figure . Gene Sequencing (extract)
  • RPB690Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • P-glycoprotein promotes epithelial T helper 2–associated cytokine secretion in chronic sinusitis with nasal polypsOnlinelibrary: alr.21316
  • P‐glycoprotein regulates Staphylococcus aureus enterotoxin B–stimulated interleukin‐5 and thymic stromal lymphopoietin secretion in organotypic mucosal explantsPubMed: 26625351
  • Intact soluble P-glycoprotein is secreted by sinonasal epithelial cells.pubmed:27456593
  • Secreted P-glycoprotein is a noninvasive biomarker of chronic rhinosinusitispubmed:27577924
  • Design of colloidal drug carriers of celecoxib for use in treatment of breast cancer and leukemia
  • Chemotherapeutic drugs stimulate the release and recycling of extracellular vesicles to assist cancer cells in developing an urgent chemoresistancePubmed: 31830995

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