Recombinant Complement Factor I (CFI) Homo sapiens (Human) Recombinant protein

CF-I; C3b-INA; FI; IF; KAF; Factor I; Konglutinogen-Activating Factor; C3b-Inactivator; C3B/C4B inactivator

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 54.3kDa, Accurate 54kDa(Analysis of differences refer to the manual)
  • Residues & TagsLeu28~Gly243 with N-terminal His and GST Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 80%
  • Isoelectric Point7.5
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  • Recombinant Complement Factor I (CFI) Packages (Simulation)
  • Recombinant Complement Factor I (CFI) Packages (Simulation)
  • Recombinant Complement Factor I (CFI) Figure . Gene Sequencing (extract)
  • RPB978Hu02.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • A case report of thrombotic microangiopathy without renal involvement carrying two novel mutations in complement-regulator genesPubMed: 26613809
  • Atypical hemolytic uremic syndrome: Korean pediatric seriesPubMed: 25443527
  • NOVEL ASSAYy2016:139118
  • Compliment factor i bioassayUS20170285024
  • A monoclonal antibody targeting amyloid β (Aβ) restores complement factor I bioactivity: Potential implications in age-related macular degeneration and …Pubmed:29782502
  • The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H33788378

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