Recombinant Epithelial Cell Adhesion Molecule (EPCAM) Chicken (Gallus) Recombinant protein

CD326; TACSTD1; EGP; KSA; M4S1; MIC18; TROP1; HEGP2; ESA; Epithelial glycoprotein; Adenocarcinoma-associated antigen; Tumor Associated Calcium Signal Transducer 1

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Molecular Mass 32.1kDa, Accurate 36kDa(Analysis of differences refer to the manual)
  • Residues & TagsAsp21~Gly260 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point6.5
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  • Recombinant Epithelial Cell Adhesion Molecule (EPCAM) Packages (Simulation)
  • Recombinant Epithelial Cell Adhesion Molecule (EPCAM) Packages (Simulation)
  • Recombinant Epithelial Cell Adhesion Molecule (EPCAM) Figure . Gene Sequencing (extract)
  • RPB283Ga01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platformScience: Article
  • Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determinationscience:S0026265X16300091
  • Role of several cytokines and adhesion molecules in the diagnosis and prediction of survival ofhepatocellular carcinoma.pubmed:27916547
  • Integrated bio-affinity nano-platform into a microfluidic immunosensor based on monoclonal bispecific trifunctional antibodies for the electrochemical determination of …pubmed:27836687
  • Integrated bio-affinity nano-platform into a microfluidic immunosensor based on monoclonal bispecific trifunctional antibodies for the electrochemical determination of epithelial cancer biomarker10.1016/j.cca.2016.11.012

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